Anti-Cytokeratin 7+17 antibody [C-46] (ab670)
![Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.com/ps/products/0/ab670/Images/ab670-3606-anti-cytokeratin-717-antibody-c-46-immunohistochemistry.jpg "Anti-Cytokeratin 7+17 antibody [C-46] (ab670)")
Key features and details
- Mouse monoclonal [C-46] to Cytokeratin 7+17
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cytokeratin 7+17 antibody [C-46] -
Description
Mouse monoclonal [C-46] to Cytokeratin 7+17 -
Host species
Mouse -
Specificity
Cytokeratin peptides 7 (54 kDa) and 17 (46 kDa) -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Rabbit -
Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 7+17. Cytoskeleton preparation of HeLa cells (cervix carcinoma)
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General notes
Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from culture supernatant. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
C-46 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Ab670 (20ug/ml) staining human Cytokeratin 7+17 in human pancreas by immunohistochemistry using formalin fixed, paraffin embedded tissue.
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![Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)](https://www.abcam.com/ps/products/0/ab670/Images/ab670-3607-anti-cytokeratin-717-antibody-c-46-western-blot.jpg)
Western blot - Anti-Cytokeratin 7+17 antibody [C-46] (ab670)Anti-Cytokeratin 7+17 antibody [C-46] (ab670) at 1 µg/ml + HeLa cell lysate
Observed band size: 49,51 kDa why is the actual band size different from the predicted?
Sample preparation:Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing conditions.)