Anti-Cytokeratin 20 antibody - C-terminal (ab230524)
Key features and details
- Rabbit polyclonal to Cytokeratin 20 - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 20 antibody - C-terminal
See all Cytokeratin 20 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 20 - C-terminal -
Host species
Rabbit -
Specificity
ab230524 recognizes endogenous levels of Cytokeratin 20 protein. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
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Immunogen
Synthetic peptide within Human Cytokeratin 20 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P35900 -
Positive control
- IHC-P: Human colon tissue. ICC/IF: A549 cells. WB: A549, NS-1, mouse and rat kidney whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 20 antibody - C-terminal (ab230524)
Formalin-fixed, paraffin-embedded human colon tissue stained for Cytokeratin 20 with ab230524 at a 1:100 dilution in immunohistochemical analysis.
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Western blot - Anti-Cytokeratin 20 antibody - C-terminal (ab230524)All lanes : Anti-Cytokeratin 20 antibody - C-terminal (ab230524) at 1/500 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : NS-1 whole cell lysate
Lane 3 : Mouse kidney whole cell lysate
Lane 4 : Rat kidney whole cell lysate
Developed using the ECL technique.
Predicted band size: 48 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 20 antibody - C-terminal (ab230524)A549 (Human lung carcinoma cell line) cells stained for Cytokeratin 20 using ab230524 at a dilution of 1/100 in ICC/IF.
Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab230524 in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
