Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab80206)
Key features and details
- Rabbit polyclonal to Cytochrome P450 17A1/CYP17A1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome P450 17A1/CYP17A1 antibody
See all Cytochrome P450 17A1/CYP17A1 primary antibodies -
Description
Rabbit polyclonal to Cytochrome P450 17A1/CYP17A1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Cytochrome P450 17A1/CYP17A1 (internal sequence) conjugated to keyhole limpet haemocyanin.
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General notes
This product was previously labelled as Cytochrome P450 17A1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab80206)Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab80206) at 1/50 dilution + K562 cell line lysates at 35 µg
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome P450 17A1/CYP17A1 antibody (ab80206)ICC/IF image of ab80206 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80206, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
