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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)

Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10782] to CYFIP1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free
    See all CYFIP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10782] to CYFIP1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, HeLa, A549, Neuro-2a, NIH3T3, C2C12, C6, PC-12 and Rat-1 cell lysates; Human brain and lung, Mouse brain, lung and breast, Rat brain and lung tissue lysates. ICC/IF: A549; Flow-cyt: 293T cells.
  • General notes

    ab249255 is the carrier-free version of ab156016.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10782
  • Isotype

    IgG
  • Research areas

    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Images

  • Western blot - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)
    Western blot - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)
    Lanes 1-7 : Anti-CYFIP1 antibody [EPR10782] (ab156016) at 1/1000 dilution (Purified)
    Lanes 8-9 : Anti-CYFIP1 antibody [EPR10782] (ab156016) at 1/1000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
    Lane 4 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 6 : C2C12 (Mouse myoblasts myoblast) whole cell lysates
    Lane 7 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 9 : Rat-1 (Rat embryonic fibroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 145 kDa
    Observed band size: 145 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156016).

  • Flow Cytometry - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)
    Flow Cytometry - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)
    Flow Cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling CYFIP1 with Purified ab156016 at 1/30 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156016).
  • Immunocytochemistry/ Immunofluorescence - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)
    Immunocytochemistry/ Immunofluorescence - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)
    Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling CYFIP1 with Purified ab156016 at 1/50 dilution (5.56 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156016).
  • Western blot - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)
    Western blot - Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)
    All lanes : Anti-CYFIP1 antibody [EPR10782] (ab156016) at 1/1000 dilution (Purified)

    Lane 1 : Human brain lysates
    Lane 2 : Human lung lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Mouse lung lysates
    Lane 5 : Mouse breast lysates
    Lane 6 : Rat brain lysates
    Lane 7 : Rat lung lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 145 kDa
    Observed band size: 145 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156016).

  • Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)
    Anti-CYFIP1 antibody [EPR10782] - BSA and Azide free (ab249255)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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