Anti-CX3CR1 antibody [EPR22354-15] - BSA and Azide free (ab267834)
![Anti-CX3CR1 antibody [EPR22354-15] - BSA and Azide free (ab267834)](https://www.abcam.com/ps/products/267/ab267834/Images/ab267834-4-anti-cx3cr1-antibody-epr22354-15-flowcytometry-pbmc-cd3-human.jpg "Anti-CX3CR1 antibody [EPR22354-15] - BSA and Azide free (ab267834)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22354-15] to CX3CR1 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CX3CR1 antibody [EPR22354-15] - BSA and Azide free
See all CX3CR1 primary antibodies -
Description
Rabbit monoclonal [EPR22354-15] to CX3CR1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Human peripheral blood mononuclear cells.
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General notes
ab267834 is the carrier-free version of ab245248. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22354-15 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CX3CR1 antibody - BSA and Azide free (ab267834)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab245248.
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Gated on viable cells and T cell panel (CD3+) is shown.
Gated on viable cells and monocytes panel (CD14+) is shown.
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Flow Cytometry - Anti-CX3CR1 antibody - BSA and Azide free (ab267834)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab245248.
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Negative control: CD19+ B cells. (PMID: 9390561).
Gated on viable cells and B cell panel (CD19+) is shown.
-
Flow Cytometry - Anti-CX3CR1 antibody - BSA and Azide free (ab267834)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab245248.
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Gated on viable cells and NK cell panel (CD56+) is shown.
-
Flow Cytometry - Anti-CX3CR1 antibody - BSA and Azide free (ab267834)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab245248.
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CX3CR1 with ab245248 at 1/600 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab245248 (Right). Then stained with anti-CD25 conjugated to BV421, anti-CD14 conjugated to BV510, anti-CD56 conjugated to PE, anti-CD19 conjugated to PE-Cy7, anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD4 conjugated to APC-FireTM750.
Gated on viable cells and monocytes panel (CD14+) is shown.
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![Anti-CX3CR1 antibody [EPR22354-15] - BSA and Azide free (ab267834)](https://www.abcam.com/ps/products/267/ab267834/Images/ab267834-5-benefits-of-recombinant-antibodies.png)
Anti-CX3CR1 antibody [EPR22354-15] - BSA and Azide free (ab267834)
