Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free
    See all Monoamine Oxidase B/MAOB primary antibodies

  • Description

    Rabbit monoclonal [EPR7102] to Monoamine Oxidase B/MAOB - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248436 is the carrier-free version of ab133270 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248436 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as Monoamine Oxidase B

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/5000 dilution (purified) + HepG2 cell lysate at 20 µg

    Secondary
    Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    This data was developed using ab133270, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/2000 dilution (purified)

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    This data was developed using ab133270, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    This data was developed using ab133270, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human liver with purified ab133270 at a working dilution of 1/300. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    Western blot - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    All lanes : Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] (ab133270) at 1/1000 dilution (Unpurified)

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : Human fetal kidney tissue lysate
    Lane 3 : Human platelet tissue lysate
    Lane 4 : Human fetal brain tissue lysate
    Lane 5 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 59 kDa



    This data was developed using ab133270, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    This data was developed using ab133270, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human brain tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100Produced under U.S. Patent No. 5,675,063.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    This data was developed using ab133270, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human kidney tissue labelling Monoamine Oxidase B/MAOB with unpurified ab133270 at 1/100 dilution.
  • OI-RD Scanning - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    OI-RD Scanning - Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    This data was developed using ab133270, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)
    Anti-Monoamine Oxidase B/MAOB antibody [EPR7102] - BSA and Azide free (ab248436)

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