Anti-CX3CR1 antibody (ab8021)
Key features and details
- Rabbit polyclonal to CX3CR1
- Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CX3CR1 antibody
See all CX3CR1 primary antibodies -
Description
Rabbit polyclonal to CX3CR1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF MouseRatHumanIHC-P MouseRatHumanWB Human
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Immunogen
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Positive control
- Western Blot: Human spleen tissue lysate, A431, A549, Caco-2, Daudi, HeLa, THP-1 cell lysates. ICC/IF: Human heart cells and K562 cells. IHC-P: Mouse, rat and human heart tissue. Flow cytometry: THP-1 cells. IF: Mouse and rat heart tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CX3CR1 antibody (ab8021)All lanes :
Lane 1 : A431 cell lysates
Lane 2 : A549 cell lysates
Lane 3 : Caco-2 cell lysates
Lane 4 : Daudi cell lysates
Lane 5 : HeLa cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Developed using the ECL technique.10% gel.
Running conditions: 130v for 2 hours.
Transfer conditions: wet, 250mA, 2 hrs (Nitrocellulose membrane).
Blocking condition: 5% non-fat dry milk in TBS, 4C, overnight.
Primary antibody incubation: Room temperature for 1 hour.
Secondary antibody incubation: Room temperature for 1 hour.
Washing conditions: 15 mL TSBT, 3 x 10 minutes.
Exposure: ECL solution
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Immunocytochemistry/ Immunofluorescence - Anti-CX3CR1 antibody (ab8021)Immunofluorescence of CX3CR1 in mouse heart tissue with ab8021 at 20 ug/ml. Green: ab8021 Blue: DAPI staining
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CR1 antibody (ab8021)Immunohistochemical analysis of paraffin-embedded rat heart tissue labeling CX3CR1 with ab8021 at 2 µg/mL.
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Flow Cytometry - Anti-CX3CR1 antibody (ab8021)Flow cytometry analysis of THP-1 cells using CX3CR1 antibody (ab8021) at 0.1 ug/ml.
Green: Isotype control
Red: CX3CR1 antibody (ab8021)
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Immunocytochemistry/ Immunofluorescence - Anti-CX3CR1 antibody (ab8021)Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed K562 cells labeling CX3CR1 with ab8021 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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Western blot - Anti-CX3CR1 antibody (ab8021)All lanes :
All lanes : THP-1 cell line
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Developed using the ECL technique.10% gel.
Running conditions: 130v for 2 hours.
Transfer conditions: wet, 250mA, 2 hrs (Nitrocellulose membrane).
Blocking condition: 5% non-fat dry milk in TBS, 4C, overnight.
Primary antibody incubation: Room temperature for 1 hour.
Secondary antibody incubation: Room temperature for 1 hour.
Washing conditions: 15 mL TSBT, 3 x 10 minutes.
Exposure: ECL solution
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Western blot - Anti-CX3CR1 antibody (ab8021)All lanes : Anti-CX3CR1 antibody (ab8021) at 1 µg/ml
Lane 1 : Human spleen lysate with absence of blocking peptide.
Lane 2 : Human spleen lysate with presence of blocking peptide.
Observed band size: 50 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CR1 antibody (ab8021)Mouse heart tissue stained for CX3CR1 using ab8021 at 2 µg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CX3CR1 antibody (ab8021)Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-CX3CR1 at 2 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-CX3CR1 antibody (ab8021)Immunofluorescence analysis of rat heart tissue labeling CX3CR1 with ab8021 at 2 µg/mL. DAPI stain in blue.
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Immunocytochemistry/ Immunofluorescence - Anti-CX3CR1 antibody (ab8021)Immunofluorescence of CX3CR1 in Human Heart cells using ab8021 at 10 ug/ml.
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Western blot - Anti-CX3CR1 antibody (ab8021)
