Anti-CREB antibody (ab31387)
Key features and details
- Rabbit polyclonal to CREB
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CREB antibody
See all CREB primary antibodies -
Description
Rabbit polyclonal to CREB -
Host species
Rabbit -
Specificity
Immunogen has 92% alignment to ATF-1 -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (Human) - non-phosphopeptide derived from human CREB around the phosphorylation site of Serine 133.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CREB antibody (ab31387) This image is courtesy of an anonymous AbreviewAll lanes : Anti-CREB antibody (ab31387) at 1/1000 dilution
Lane 1 : Mouse primary hippocampal neurons whole cell lysate
Lane 2 : Rat primary hippocampal neurons whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody (ab31387)Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 ab31387. Left: Untreated sample; Right: Sample treated with synthesized peptide (negative control). -
Western blot - Anti-CREB antibody (ab31387)Lane 1 : Anti-CREB antibody (ab31387) at 1/500 dilution
Lane 2 : Anti-CREB antibody (ab31387) at 1/500 dilution (treated with synthesized peptide )
All lanes : HeLa cell extract
Predicted band size: 37 kDa
