Anti-CPM antibody [EPR8052] (ab150405)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8052] to CPM
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CPM antibody [EPR8052]
See all CPM primary antibodies -
Description
Rabbit monoclonal [EPR8052] to CPM -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human CPM aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: Recombinant Human CPM protein (ab132341), 293T cell lysate and human fetal kidney, fetal liver and placenta tissue lysates. IHC-P: Human hepatocellular carcinoma, endometrium and uterus tissues.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8052 -
Isotype
IgG -
Research areas
Images
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Anti-CPM antibody [EPR8052] (ab150405) at 1/2500 dilution (purified) + Human placenta tissue lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-CPM antibody [EPR8052] (ab150405) at 1/5000 dilution (purified)
Lane 1 : Human fetal liver tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] (ab150405)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling CPM with purified ab150405 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-CPM antibody [EPR8052] (ab150405) at 1/1000 dilution (unpurified)
Lane 1 : 293T lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal
liver lysate
Lane 4 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] (ab150405)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling CPM with unpurified ab150405 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPM antibody [EPR8052] (ab150405)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus tissue labelling CPM with unpurified ab150405 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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