Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µg 268 032 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Rat monoclonal [JOR110A] to SAA3
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Mouse, Human
Isotype: IgG2b

Product name

Anti-SAA3 antibody [JOR110A]
See all SAA3 primary antibodies
Description

Rat monoclonal [JOR110A] to SAA3
Host species

Rat

Tested Applications & Species

Application	Species
IHC-Fr	Human
IHC-P	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment (His-tag) corresponding to Mouse SAA3 aa 1 to the C-terminus.
Database link: P04918

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Positive control
- WB; RAW12 cells stimulated with LPS for 8, 12, 16 and 24 hours, supernatant; mouse SAA3-transfected HEK-293T supernatant. IHC-P: Mouse colitis, colon, adenocarcinoma, mesothelium, pneumonia and lung tissue; adipocytes in mouse adenocarcinoma tissue. IHC-Fr: Frozen cytospin preparation of MYC-tagged SAA3-transfected HEK-293T.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Clonality

Monoclonal
Clone number

JOR110A
Isotype

IgG2b

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Paraffin-embedded mouse colitis tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Paraffin-embedded mouse colon tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Paraffin-embedded mouse adenocarcinoma tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Paraffin-embedded mouse mesothelium tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Paraffin-embedded mouse pneumonia tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Paraffin-embedded mouse lung tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Paraffin-embedded mouse adipocytes in adenocarcinoma tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Paraffin-embedded mouse adepocytes in SAA KO mouse stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Frozen cytospin preparation of MYC-tagged SAA1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Frozen cytospin preparation of MYC-tagged SAA2-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis (negative control).
Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Frozen MYC-tagged SAA3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cytospin stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

Frozen cytospin preparation of MYC-tagged SAA4-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis (negative control).
Western blot - Anti-SAA3 antibody [JOR110A] (ab231680)

All lanes : Anti-SAA3 antibody [JOR110A] (ab231680) at 1/50 dilution

Lane 1 : RAW12 stimulated with LPS for 8 hours supernatant at 20 µl
Lane 2 : RAW12 stimulated with LPS for 12 hours supernatant at 20 µl
Lane 3 : RAW12 stimulated with LPS for 16 hours supernatant at 20 µl
Lane 4 : RAW12 stimulated with LPS for 24 hours supernatant at 20 µl
Lane 5 : RAW12 supernatant at 20 µl
Lane 6 : Mouse SAA3-GFP-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Developed using the ECL technique.

Predicted band size: 14 kDa
Western blot - Anti-SAA3 antibody [JOR110A] (ab231680)

All lanes : Anti-SAA3 antibody [JOR110A] (ab231680) at 1/50 dilution

Lane 1 : SAA1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 2 : SAA2-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 3 : SAA3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 4 : SAA4-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 5 : RAW12 supernatant at 20 µl
Lane 6 : RAW12 stimulated with LPS for 12 hours supernatant at 20 µl

Developed using the ECL technique.

Predicted band size: 14 kDa

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