Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAA3 antibody [JOR110A] (ab231680)

Key features and details

  • Rat monoclonal [JOR110A] to SAA3
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-SAA3 antibody [JOR110A]
    See all SAA3 primary antibodies

  • Description

    Rat monoclonal [JOR110A] to SAA3

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Human
    IHC-P
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment (His-tag) corresponding to Mouse SAA3 aa 1 to the C-terminus.
    Database link: P04918

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB; RAW12 cells stimulated with LPS for 8, 12, 16 and 24 hours, supernatant; mouse SAA3-transfected HEK-293T supernatant. IHC-P: Mouse colitis, colon, adenocarcinoma, mesothelium, pneumonia and lung tissue; adipocytes in mouse adenocarcinoma tissue. IHC-Fr: Frozen cytospin preparation of MYC-tagged SAA3-transfected HEK-293T.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    JOR110A

  • Isotype

    IgG2b

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Paraffin-embedded mouse colitis tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Paraffin-embedded mouse colon tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Paraffin-embedded mouse adenocarcinoma tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Paraffin-embedded mouse mesothelium tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Paraffin-embedded mouse pneumonia tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Paraffin-embedded mouse lung tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Paraffin-embedded mouse adipocytes in adenocarcinoma tissue stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Paraffin-embedded mouse adepocytes in SAA KO mouse stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Frozen cytospin preparation of MYC-tagged SAA1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Frozen cytospin preparation of MYC-tagged SAA2-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis (negative control).

  • Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Frozen MYC-tagged SAA3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cytospin stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)
    Immunohistochemistry (Frozen sections) - Anti-SAA3 antibody [JOR110A] (ab231680)

    Frozen cytospin preparation of MYC-tagged SAA4-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) stained for SAA3 using ab231680 at 1/50 dilution in immunohistochemical analysis (negative control).

  • Western blot - Anti-SAA3 antibody [JOR110A] (ab231680)
    Western blot - Anti-SAA3 antibody [JOR110A] (ab231680)
    All lanes : Anti-SAA3 antibody [JOR110A] (ab231680) at 1/50 dilution

    Lane 1 : RAW12 stimulated with LPS for 8 hours supernatant at 20 µl
    Lane 2 : RAW12 stimulated with LPS for 12 hours supernatant at 20 µl
    Lane 3 : RAW12 stimulated with LPS for 16 hours supernatant at 20 µl
    Lane 4 : RAW12 stimulated with LPS for 24 hours supernatant at 20 µl
    Lane 5 : RAW12 supernatant at 20 µl
    Lane 6 : Mouse SAA3-GFP-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

    Developed using the ECL technique.

    Predicted band size: 14 kDa

  • Western blot - Anti-SAA3 antibody [JOR110A] (ab231680)
    Western blot - Anti-SAA3 antibody [JOR110A] (ab231680)
    All lanes : Anti-SAA3 antibody [JOR110A] (ab231680) at 1/50 dilution

    Lane 1 : SAA1-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
    Lane 2 : SAA2-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
    Lane 3 : SAA3-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
    Lane 4 : SAA4-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
    Lane 5 : RAW12 supernatant at 20 µl
    Lane 6 : RAW12 stimulated with LPS for 12 hours supernatant at 20 µl

    Developed using the ECL technique.

    Predicted band size: 14 kDa

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