Human IL-13 ELISPOT Kit (without plates) (ab46580)
Key features and details
- Assay type: Sandwich (qualitative)
- Sample type: Suspension cells
- Reacts with: Human
Overview
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Product name
Human IL-13 ELISPOT Kit (without plates) -
Sample type
Suspension cells -
Assay type
Sandwich (qualitative) -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Elispot assay is designed to enumerate cytokine producing cells in a single cell suspension. This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. Elispot assay constitutes an ideal tool in the TH1 / TH2 response, vaccine development, viral infection monitoring and treatment, cancerology, infectious diseases, autoimmune diseases and transplantation.
This Elispot assay is based on sandwich immuno-enzyme technology. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Principal:
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Recognizes both natural and recombinant human IL-13
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Tested applications
Suitable for: ELISpotmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 15 x 96 tests 20 x 96 tests Bovine Serum Albumin 1 x 1g 2 x 1g 3 x 1g 4 x 1g Dry Skimmed milk 1 vial 2 vials 3 vials 4 vials Clone B-P6 Biotinylated detection antibody, 1 vial 2 vials 3 vials 4 vials Clone B-B13 Capture antibody 1 x 500µl 2 x 500µl 3 x 500µl 4 x 500µl Ready-to-use BCIP/NBT substrate buffer 1 x 50ml 2 x 50ml 3 x 50ml 4 x 50ml Streptavidin - Alkaline Phosphatase conjugated 1 x 50µl 2 x 50µl 3 x 50µl 4 x 50µl -
Research areas
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Relevance
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. It is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. IL-13, IL-3, IL-5, IL-4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL-4. -
Cellular localization
Secreted -
Alternative names
- ALRH
- BHR1
- IL 13
see all -
Database links
- Entrez Gene: 3596 Human
- Omim: 147683 Human
- SwissProt: P35225 Human
- Unigene: 845 Human
