Lysozyme Activity Assay Kit (Fluorometric) (ab211113)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Cell Lysate, Purified protein, Saliva, Serum, Tissue Homogenate, Tissue Lysate
- Sensitivity: 0.002 U/ml
Overview
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Product name
Lysozyme Activity Assay Kit (Fluorometric)
See all Lysozyme kits -
Detection method
Fluorescent -
Sample type
Saliva, Serum, Cell culture extracts, Cell Lysate, Tissue Homogenate, Purified protein, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
0.002 U/ml -
Product overview
Lysozyme Activity Assay Kit (ab211113) provides a convenient method for quantifying Lysozyme activity in mammalian cell or tissue lysates, biological fluids such as tears or saliva. This product can also be used to measure lysozyme activity in yeast and bacteria. The assay is based on the ability of lysozyme to cleave a synthetic substrate and release a free fluorophore which can be easily quantified at Ex/Em= 360/445 nm in a fluorescent microplate reader.
This kit provides a simple, ultra-sensitive assay that can detect as low as 2 µU/mL of Lysozyme activity.
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Notes
Lysozyme (EC 3.2.1.17), also known as muramidase or N-acetylmuramide glycanhydrolase, is a hydrolase acting on glycosidic bonds. It hydrolyzes the β-(1-4)-glucosidic linkage between N-acetyl-muraminic acid and N-acetyl-D-glucosamine residues present in the mucopolysaccharide cell wall. Lysozyme is ubiquitously found in a wide range of biological fluids such as tears, saliva and tissues. It exhibits antibacterial, antitumor and immune modulatory activities. Elevated concentrations of lysozyme in urine and serum have been reported in patients suffering leukemia, tuberculosis, megaloblastic anemias, acute bacterial infections, ulcerative colitis, severe renal insufficiency, pyelonephritis and nephritis.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Lysozyme Assay Buffer 1 x 25ml Lysozyme Stop Buffer 1 x 25ml Lysozyme Substrate (in DMSO) 1 x 65µl Lysozyme Positive Control (200 U) 1 vial 4-Methylumbelliferone Standard (5 mM) 1 x 35µl -
Research areas
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Function
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
Involvement in disease
Amyloidosis 8 -
Sequence similarities
Belongs to the glycosyl hydrolase 22 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 1
- 1 4 beta N acetylmuramidase C
- 4-beta-N-acetylmuramidase C
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Images
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Lysozyme Activity Assay Kit (Fluorometric) (ab211113)
Typical 4-Methylumbelliferon (4-MU) Standard Curve.
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Lysozyme Activity Assay Kit (Fluorometric) (ab211113)Measurement of Lysozyme activity in human fluids. Assays were performed following kit protocols.
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Lysozyme Activity Assay Kit (Fluorometric) (ab211113)Measurement of Lysozyme activity in human cultured cell lysates. Assays were performed following kit protocols.