Anti-CPEB3 antibody (ab10883)
Key features and details
- Rabbit polyclonal to CPEB3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CPEB3 antibody -
Description
Rabbit polyclonal to CPEB3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Mouse CPEB3 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab24478)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab10883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Sequence similarities
Belongs to the RRM CPEB family.
Contains 2 RRM (RNA recognition motif) domains. - Information by UniProt
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Database links
- Entrez Gene: 22849 Human
- Entrez Gene: 208922 Mouse
- SwissProt: Q8NE35 Human
- SwissProt: Q7TN99 Mouse
- Unigene: 131683 Human
- Unigene: 701732 Human
- Unigene: 371677 Mouse
- Unigene: 391176 Mouse
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Alternative names
- CPE-binding protein 3 antibody
- CPE-BP3 antibody
- CPEB 3 antibody
see all
Images
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Western blot - Anti-CPEB3 antibody (ab10883)Lane 1 : Anti-CPEB3 antibody (ab10883) at 1/500 dilution
Lane 2 : Anti-CPEB3 antibody (ab10883) at 1/1000 dilution
All lanes : Mouse Brain lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
Predicted band size: 75 kDa
Exposure time: 3 minutesLanes 1 to 2
Lane 1: 5µ
Lane 2: 5µ
Exposure time: 3 minutes
Expected molecular weight: 78kDa
Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
ab7090 (1/5000)We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.
We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature. -
Immunocytochemistry/ Immunofluorescence - Anti-CPEB3 antibody (ab10883)ICC/IF image of ab10883 stained SK-N-SH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10883 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References (9)
ab10883 has been referenced in 9 publications.
- Pascual R et al. The RNA binding protein CPEB2 regulates hormone sensing in mammary gland development and luminal breast cancer. Sci Adv 6:eaax3868 (2020). PubMed: 32440535
- Chen Y et al. Long non-coding RNA HCG11 modulates glioma progression through cooperating with miR-496/CPEB3 axis. Cell Prolif 52:e12615 (2019). PubMed: 31310044
- Parras A et al. Autism-like phenotype and risk gene mRNA deadenylation by CPEB4 mis-splicing. Nature 560:441-446 (2018). PubMed: 30111840
- Margvelani G et al. Micro-RNAs, their target proteins, predispositions and the memory of filial imprinting. Sci Rep 8:17444 (2018). PubMed: 30487553
- Kaczmarczyk L et al. New Phosphospecific Antibody Reveals Isoform-Specific Phosphorylation of CPEB3 Protein. PLoS One 11:e0150000 (2016). WB . PubMed: 26915047
- Burguete AS et al. GGGGCC microsatellite RNA is neuritically localized, induces branching defects, and perturbs transport granule function. Elife 4:e08881 (2015). PubMed: 26650351
- Pedraza N et al. KIS, a kinase associated with microtubule regulators, enhances translation of AMPA receptors and stimulates dendritic spine remodeling. J Neurosci 34:13988-97 (2014). WB, IP ; Mouse . PubMed: 25319695
- La Via L et al. Modulation of dendritic AMPA receptor mRNA trafficking by RNA splicing and editing. Nucleic Acids Res 41:617-31 (2013). ICC/IF ; Rat . PubMed: 23166306
- Wang XP & Cooper NG Characterization of the transcripts and protein isoforms for cytoplasmic polyadenylation element binding protein-3 (CPEB3) in the mouse retina. BMC Mol Biol 10:109 (2009). PubMed: 20003455
Images
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Western blot - Anti-CPEB3 antibody (ab10883)Lane 1 : Anti-CPEB3 antibody (ab10883) at 1/500 dilution
Lane 2 : Anti-CPEB3 antibody (ab10883) at 1/1000 dilution
All lanes : Mouse Brain lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
Predicted band size: 75 kDa
Exposure time: 3 minutesLanes 1 to 2
Lane 1: 5µ
Lane 2: 5µ
Exposure time: 3 minutes
Expected molecular weight: 78kDa
Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
ab7090 (1/5000)We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.
We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature. -
Immunocytochemistry/ Immunofluorescence - Anti-CPEB3 antibody (ab10883)
ICC/IF image of ab10883 stained SK-N-SH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10883 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
