Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Cleaved PARP1 antibody [PARP-H8] (ab278604)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [PARP-H8] to Cleaved PARP1
Suitable for: Flow Cyt, WB
Reacts with: Human

Product name

Anti-Cleaved PARP1 antibody [PARP-H8]
See all Cleaved PARP1 primary antibodies
Description

Rabbit monoclonal [PARP-H8] to Cleaved PARP1
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Cleaved PARP1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P09874

Run BLAST with Run BLAST with
Positive control
- WB: HeLa (treated with staurosporine) cell extract. Flow cyt: SK-N0MC cells (treated with staurosporine).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

PARP-H8
Isotype

IgG
Light chain type

kappa
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- PARP
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- PARP

Flow Cytometry - Anti-Cleaved PARP1 antibody [PARP-H8] (ab278604)

Flow cytometric analysis of SK.N.MC cells unstained untreated cells as negative control (blue) or stained untreated (red) or treated with staurosporine (green) using ab278604 at 0.1 µg/mL.
Western blot - Anti-Cleaved PARP1 antibody [PARP-H8] (ab278604)

All lanes : Anti-Cleaved PARP1 antibody [PARP-H8] (ab278604) at 0.001 µg/ml

Lane 1 : HeLa cell extract treated with staurosporine
Lane 2 : HeLa cell extract untreated

Predicted band size: 113 kDa
Flow Cytometry - Anti-Cleaved PARP1 antibody [PARP-H8] (ab278604)

Peptide blocking flow cytometric analysis of SK.N.MC cells secondary antibody only negative control (light blue) or untreated (red) or treated with staurosporine (green) or untreated and blocked with immunogen peptide (black) or treated and blocked with immunogen peptide (gold) or untreated and blocked with irrelevant peptide (dark blue) or treated and blocked with irrelevant peptide (purple) using ab278604 at 0.1 µg/mL.
Flow Cytometry - Anti-Cleaved PARP1 antibody [PARP-H8] (ab278604)

Flow cytometric analysis of SK.N.MC cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using ab278604 at 0.1 µg/mL.
Anti-Cleaved PARP1 antibody [PARP-H8] (ab278604)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com