Anti-CEP290 antibody (ab85728)
Key features and details
- Rabbit polyclonal to CEP290
- Suitable for: ICC, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CEP290 antibody
See all CEP290 primary antibodies -
Description
Rabbit polyclonal to CEP290 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide corresponding to a region between residue 2429 and 2479 of Human CEP290 (NP_079390.3).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CEP290 antibody (ab85728)All lanes : Anti-CEP290 antibody (ab85728) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 290 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 200 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunocytochemistry - Anti-CEP290 antibody (ab85728)Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling CEP290 with ab85728 at 1/500. A DyLight® 594-conjugated goat anti-rabbit IgG (1/100) was used as the secondary antibody. -
Immunocytochemistry - Anti-CEP290 antibody (ab85728)ICC/IF image of ab85728 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85728, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunoprecipitation - Anti-CEP290 antibody (ab85728)Detection of Human CEP290 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85728 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
