Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6650(2)] to Cdk7 (phospho T170)
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cdk7 (phospho T170) antibody [EPR6650(2)]
    See all Cdk7 primary antibodies

  • Description

    Rabbit monoclonal [EPR6650(2)] to Cdk7 (phospho T170)

  • Host species

    Rabbit

  • Specificity

    This antibody only detects CDK7 phosphorylated at threonine 170.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cdk7 (phospho T170).
    Database link: P50613

  • Positive control

    • WB: HeLa, A431, NIH/3T3 and C6 cell lysates and rat spleen tissue lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified)

    Lane 1 : HeLa cell lysate (untreated)
    Lane 2 : HeLa cell lysate (treated with Lambda phosphatase)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified) + A431 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified) + NIH/3T3 cell lysate at 20 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified)

    Lane 1 : C6 cell lysate
    Lane 2 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate (untreated)
    Lane 2 : HeLa cell lysate (treated with Lambda phosphatase)

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 39 kDa

  • Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
    Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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