Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
![Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)](https://www.abcam.com/ps/products/155/ab155976/Images/ab155976-240883-anti-cdk7-phospho-t170-antibody-epr66502-western-blot.jpg "Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6650(2)] to Cdk7 (phospho T170)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cdk7 (phospho T170) antibody [EPR6650(2)]
See all Cdk7 primary antibodies -
Description
Rabbit monoclonal [EPR6650(2)] to Cdk7 (phospho T170) -
Host species
Rabbit -
Specificity
This antibody only detects CDK7 phosphorylated at threonine 170. -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cdk7 (phospho T170).
Database link: P50613 -
Positive control
- WB: HeLa, A431, NIH/3T3 and C6 cell lysates and rat spleen tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6650(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified)
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with Lambda phosphatase)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)](https://www.abcam.com/ps/products/155/ab155976/Images/ab155976-240884-anti-cdk7-phospho-t170-antibody-epr66502-western-blot.jpg)
Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified) + A431 cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)](https://www.abcam.com/ps/products/155/ab155976/Images/ab155976-240885-anti-cdk7-phospho-t170-antibody-epr66502-western-blot.jpg)
Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified) + NIH/3T3 cell lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)](https://www.abcam.com/ps/products/155/ab155976/Images/ab155976-240886-anti-cdk7-phospho-t170-antibody-epr66502-western-blot.jpg)
Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified)
Lane 1 : C6 cell lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
![Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)](https://www.abcam.com/ps/products/155/ab155976/Images/ab155976-163282-anti-cdk7-phospho-t170-antibody-epr66502-western-blot.jpg)
Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate (treated with Lambda phosphatase)
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa
-
![Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)](https://www.abcam.com/ps/products/155/ab155976/Images/ab155976-6-benefits-of-recombinant-antibodies.png)
Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)
