Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cdk7 antibody [BL-80-3D4] (ab243863)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BL-80-3D4] to Cdk7
Suitable for: ICC, IP, IHC-P, WB
Reacts with: Human

Product name

Anti-Cdk7 antibody [BL-80-3D4]
See all Cdk7 primary antibodies
Description

Rabbit monoclonal [BL-80-3D4] to Cdk7
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cdk7 aa 300-346 (C terminal). The exact sequence is proprietary. Gene ID 1022.
Database link: P50613
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BL-80-3D4
Isotype

IgG
Research areas

Immunocytochemistry - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)

Formaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling brd4 using ab243863 at 1/100 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for cdk7 using ab243863 at 1/30 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Immunoprecipitation - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)

cdk7 was immunoprecipitated from 0.5 mg HeLa whole cell lysate with ab243863 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243863 at 1/1000 dilution.
Lane 1: ab243863 IP in HeLa cell lysate.

Lane 2: Contol IgG in HeLa cell lysate.
Western blot - Anti-Cdk7 antibody [BL-80-3D4] (ab243863)

Western blot analysis using ab243863 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (15 µg).
Lane 2: HEK-293T whole cell lysate (15 µg).
Lane3: Jurkat whole cell lysate (15 µg).
Lane 4: MCF-7 whole cell lysate (15 µg).
Lane 5: HepG2 whole cell lysate (15 µg).
Lane 6: A549 whole cell lysate (15 µg).
Lane 7: SW620 whole cell lysate (15 µg).
Lane 8: SK whole cell lysate (15 µg).
Lane 9: HCT 116 whole cell lysate (15 µg).
a HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds
Anti-Cdk7 antibody [BL-80-3D4] (ab243863)

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