Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17621] to CDK2 (phospho T160) + CDK1 (phospho T161)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621]
See all CDK2 (phospho T160) + CDK1 (phospho T161) primary antibodies -
Description
Rabbit monoclonal [EPR17621] to CDK2 (phospho T160) + CDK1 (phospho T161) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17621 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CDK2 (phospho T160) + CDK1 (phospho T161) antibody [EPR17621] (ab183554) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysates
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates treated with 40 ng/ml nocodazole for 24 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
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Dot blot analysis of CDK2 (phospho T160) phospho peptide (Lane 1) and CDK2 non peptide (Lane 2) using ab183554 at 1/1000 dilution detecting CDK2 (phospho T160) phospho peptide only. A Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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Dot blot analysis of CDK1 (phospho T161) phospho peptide (Lane 1) and CDK1 non peptide (Lane 2) using ab183554 at 1/1000 dilution detecting CDK1 (phospho T161) phospho peptide only. A Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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