Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22644-79] to Semaphorin 7a - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free
    See all Semaphorin 7a primary antibodies

  • Description

    Rabbit monoclonal [EPR22644-79] to Semaphorin 7a - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil and placenta tissues. Flow Cyt: Human peripheral blood mononuclear cells. IP: Human testis lysate.

  • General notes

    Ab256812 is the carrier-free version of ab255602. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256812 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)
    Immunoprecipitation - Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)

    Semaphorin 7a was immunoprecipitated from 0.35 mg human testis lysate 10µg with ab255602 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255602. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at a 1/1000 dilution.

    Lane 1: Human testis lysate 10µg
    Lane 2: ab255602 IP in Human testis lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255602 in human testis lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255602).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Semaphorin 7a with ab255602 at 1/100 dilution (6.46µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta. The section was incubated with ab255602 for 15 mins at room temperature.  The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255602).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Semaphorin 7a with ab255602 at 1/100 dilution (6.46 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab255602 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255602).

  • Flow Cytometry - Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)
    Flow Cytometry - Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)

    Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) treated with 10μg/ml Phytohemagglutinin (PHA) for 2 days (Right pane;) / Untreated control (Left panel) labeling Semaphorin 7a with ab255602 at a 1/600 dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) secondary antibody was used at a 1/5000 dilution. Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255602).

  • Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)
    Anti-Semaphorin 7a antibody [EPR22644-79] - BSA and Azide free (ab256812)

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