Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Semaphorin 7a antibody [EPR22644-316] (ab263900)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22644-316] to Semaphorin 7a
Suitable for: ICC/IF, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-Semaphorin 7a antibody [EPR22644-316]
See all Semaphorin 7a primary antibodies
Description

Rabbit monoclonal [EPR22644-316] to Semaphorin 7a
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human cerebral cortex, tonsil and liver tissues. ICC/IF: Human PBMC cells treated with phytohaemagglutinin.Flow Cyt: Human peripheral blood mononuclear cells treated with phytohaemagglutinin.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22644-316
Isotype

IgG
Research areas

Flow Cytometry - Anti-Semaphorin 7a antibody [EPR22644-316] (ab263900)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) treated with 10µg/ml phytohemagglutinin (PHA) for 2 days labeling Semaphorin 7a with ab263900 at 1\500 compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells.
Immunocytochemistry/ Immunofluorescence - Anti-Semaphorin 7a antibody [EPR22644-316] (ab263900)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labeling Semaphorin 7a with ab263900 at 1/250 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in human PBMC cells treated with phytohaemagglutinin (10 μg/ml) for 2 days. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 7a antibody [EPR22644-316] (ab263900)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Semaphorin 7a with ab263900 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Low expression tissue: Faint cytoplasmic staining on human liver. The section was incubated with ab263900 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 7a antibody [EPR22644-316] (ab263900)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Semaphorin 7a with ab263900 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil is observed. The section was incubated with ab263900 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 7a antibody [EPR22644-316] (ab263900)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling Semaphorin 7a with ab263900 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebral cortex is observed. The section was incubated with ab263900 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-Semaphorin 7a antibody [EPR22644-316] (ab263900)

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