Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Semaphorin 7a antibody [EPR22644-79] (ab255602)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22644-79] to Semaphorin 7a
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Human

Product name

Anti-Semaphorin 7a antibody [EPR22644-79]
See all Semaphorin 7a primary antibodies
Description

Rabbit monoclonal [EPR22644-79] to Semaphorin 7a
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Untreated A549, A549 treated with TNF-alpha; human tonsil, testis and placenta lysates. IHC-P: Human tonsil and placenta tissue. Flow Cyt: Human peripheral blood mononuclear cells. IP: Human testis lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22644-79
Isotype

IgG
Research areas

Western blot - Anti-Semaphorin 7a antibody [EPR22644-79] (ab255602)

All lanes : Anti-Semaphorin 7a antibody [EPR22644-79] (ab255602) at 1/1000 dilution

Lane 1 : Human tonsil lysate
Lane 2 : Human testis lysate
Lane 3 : Human placenta lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 75 kDa

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26752048, 20962327).

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 10 secs; Lanes 2-3: 37 secs.
Western blot - Anti-Semaphorin 7a antibody [EPR22644-79] (ab255602)

All lanes : Anti-Semaphorin 7a antibody [EPR22644-79] (ab255602) at 1/1000 dilution

Lane 1 : Untreated A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : A549 treated with 100ng/ml TNF-alpha for 8 hours whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 75 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26752048, 20962327).
Immunoprecipitation - Anti-Semaphorin 7a antibody [EPR22644-79] (ab255602)

Semaphorin 7a was immunoprecipitated from 0.35 mg human testis lysate 10µg with ab255602 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255602. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at a 1/1000 dilution.

Lane 1: Human testis lysate 10µg
Lane 2: ab255602 IP in Human testis lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255602 in human testis lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 7a antibody [EPR22644-79] (ab255602)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Semaphorin 7a with ab255602 at 1/100 dilution (6.46µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta. The section was incubated with ab255602 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Semaphorin 7a antibody [EPR22644-79] (ab255602)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Semaphorin 7a with ab255602 at 1/100 dilution (6.46 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil. The section was incubated with ab255602 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Flow Cytometry - Anti-Semaphorin 7a antibody [EPR22644-79] (ab255602)

Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) treated with 10μg/ml Phytohemagglutinin (PHA) for 2 days (Right pane;) / Untreated control (Left panel) labeling Semaphorin 7a with ab255602 at a 1/600 dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150097) secondary antibody was used at a 1/5000 dilution. Gated on viable cells.
Anti-Semaphorin 7a antibody [EPR22644-79] (ab255602)

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