Anti-Cdc27 antibody (ab72217)
Key features and details
- Rabbit polyclonal to Cdc27
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cdc27 antibody
See all Cdc27 primary antibodies -
Description
Rabbit polyclonal to Cdc27 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 774 and 824 of human Cdc27 (NP_001247.2).
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Positive control
- Whole cell lysates from HeLa and 293T cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cdc27 antibody (ab72217)All lanes : Anti-Cdc27 antibody (ab72217) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 55 kDa, 60 kDa, 68 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-Cdc27 antibody (ab72217)Detection of Human Cdc27 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72217 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
