Anti-Cdc27 (phospho T244) antibody (ab12281)
Key features and details
- Rabbit polyclonal to Cdc27 (phospho T244)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cdc27 (phospho T244) antibody
See all Cdc27 primary antibodies -
Description
Rabbit polyclonal to Cdc27 (phospho T244) -
Host species
Rabbit -
Specificity
ab12281 is specific for the phosphorylated form of the Cdc27 (phospho T244). Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. The antibody does not cross-react with Cdc27 phosphorylated at other sites. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide: C-VISPDTpVPLGT conjugated to KLH, corresponding to amino acids 240-248 of Human cdc27.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cdc27 (phospho T244) antibody (ab12281)All lanes : Anti-Cdc27 (phospho T244) antibody (ab12281) at 1/500 dilution
Lane 1 : HeLa whole cell lysate from asynchronous cells
Lane 2 : HeLa whole cell lysate from cells treated with nocodazole
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG [H&L] MX at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 92 kDa why is the actual band size different from the predicted?Phosphorylated Cdc27 is mostly present only in cell preparations arrested in mitosis.
