Anti-Bax antibody (ab263897)
Key features and details
- Rabbit polyclonal to Bax
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bax antibody
See all Bax primary antibodies -
Description
Rabbit polyclonal to Bax -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Bax (N terminal). The exact sequence is proprietary. Carrier-protein conjugated
Database link: Q07812 -
Positive control
- WB: HepG2 cells; ICC/IF: HeLa cells; IHC-P: Human lung carcinoma; IP: MCF-7 cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bax antibody (ab263897)Anti-Bax antibody (ab263897) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 21 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody (ab263897)Immunocsytochemistry/Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labelling Bax with ab263897 at 1/400 dilution (green). Hoechst 33342 was used as a nuclear counterstain (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (ab263897)Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labelling Bax with ab263897 at 1/500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Immunoprecipitation - Anti-Bax antibody (ab263897)Bax was immunoprecipitated from MCF-7 whole cell lysate with 4μg ab263897. Western blot was performed on the immunoprecipitate using Ab263897 at 1/500 dilution. EasyBlot anti-rabbit IgG was used as the secondary antibody. 15% SDS PAGE.
Lane 1: MCF-7 whole cell lysate control with 4ug of preimmune Rabbit IgG
Lane 2: ab263897 IP in MCF-7 whole cell lysate
