Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)
![Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)](https://www.abcam.com/ps/products/270/ab270752/Images/ab270752-383493-anticd94antibodyepr23229138flowcytometryjurkat.jpg "Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23229-138] to CD94 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD94 antibody [EPR23229-138] - BSA and Azide free
See all CD94 primary antibodies -
Description
Rabbit monoclonal [EPR23229-138] to CD94 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: NK-92 cell. Flow Cyt: Jurkat cell.
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General notes
ab270752 is the carrier-free version of ab245240. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23229-138 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell, Right) cells labelling CD94 with ab245240 at 1/500 dilution (0.1ug)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID: 9034158)
Gated on viable cells.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245240).
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![Immunocytochemistry/ Immunofluorescence - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)](https://www.abcam.com/ps/products/270/ab270752/Images/ab270752-1-anti-cd94-antibody-epr23229138-immunocytochemistry-nk92.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NK-92 cells labelling CD94 with ab245240 at 1/100 5 ug/ml dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in NK-92 cell line is observed.
Negative control: Jurkat PMID: 9034158.Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245240).
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![Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)](https://www.abcam.com/ps/products/270/ab270752/Images/ab270752-19-benefits-of-recombinant-antibodies.png)
Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)
