Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23229-138] to CD94 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD94 antibody [EPR23229-138] - BSA and Azide free
    See all CD94 primary antibodies

  • Description

    Rabbit monoclonal [EPR23229-138] to CD94 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: NK-92 cell. Flow Cyt: Jurkat cell.

  • General notes

    ab270752 is the carrier-free version of ab245240. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23229-138

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)
    Flow Cytometry - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)

    Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell, Right) cells labelling CD94 with ab245240 at 1/500 dilution (0.1ug)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID: 9034158)
    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245240).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)
    Immunocytochemistry/ Immunofluorescence - Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NK-92 cells labelling CD94 with ab245240 at 1/100 5 ug/ml dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous staining in NK-92 cell line is observed.


    Negative control: Jurkat PMID: 9034158.

    Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245240).

  • Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)
    Anti-CD94 antibody [EPR23229-138] - BSA and Azide free (ab270752)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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