Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22409-124] to CD16 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD16 antibody [EPR22409-124] - BSA and Azide free
    See all CD16 primary antibodies

  • Description

    Rabbit monoclonal [EPR22409-124] to CD16 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human spleen and colon cancer lysates. IP: Human spleen lysate. IHC-P: Human spleen and liver tissue. ICC/IF: Human PBMCs. Flow cyt: Human PBMCs.

  • General notes

    ab252908 is the carrier-free version of ab246222.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)
    Immunoprecipitation - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)

    CD16 was immunoprecipitated from 0.35 mg of human spleen lysate with ab246222 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab246222 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human spleen lysate 10 μg (Input). 

    Lane 2: ab246222 IP in human spleen lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246222 in human spleen lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Flow Cytometry - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)
    Flow Cytometry - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)

    Human PBMCs (human peripheral blood mononuclear cells) were stained with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)(Left) or ab246222 (Right) followed by a Goat anti rabbit IgG (Dylight ® 488) at 1/2000 dilution. They were then stained with Alexa Fluor® 647-conjugated anti-CD14. The expression pattern is consistent with what described in the literature. (PMID: 21738687)

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Flow Cytometry - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)
    Flow Cytometry - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)

    Negative control: Raji. (PMID:11207281).

    Raji (human Burkitt's lymphoma cell line) were stained with Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)(Left) or ab246222 (Right) followed by a Goat anti rabbit IgG (Dylight ® 488) at 1/2000 dilution.  

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD16 with ab246222 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kuffer cells of human liver (PMID: 26512139) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab246222 for 10 minutes at 37?. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD16 with ab246222 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red pulp of human spleen (PMID: 26512139; 29692344). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab246222 for 10 minutes at 37ºC. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)
    Immunocytochemistry/ Immunofluorescence - Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMCs (human peripheral blood mononuclear cells) or Raji (human Burkitt's lymphoma cell line) cell line labeling CD16 with ab246222 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of human PBMC cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Negative control: Raji (PMID:11207281).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246222).

  • Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)
    Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD16 antibody [EPR22409-124] - BSA and Azide free (ab252908)

© 2021 Astana Biomed Group LLP. All rights reserved.