Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)
![Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)](https://www.abcam.com/ps/products/249/ab249663/Images/ab249663-406434-anti-qprt-antibody-epr11941-b-bsa-and-azide-free-western-blot.jpg "Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11941(B)] to QPRT - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free
See all QPRT primary antibodies -
Description
Rabbit monoclonal [EPR11941(B)] to QPRT - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249663 is the carrier-free version of ab171944. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249663 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11941(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)
This data was developed using ab171944, the same antibody clone in a different buffer formulation.
Lane 1 Wild-type HAP1 cell lysate (20 µg)
Lane 2 QPRT knockout HAP1 cell lysate (20 µg)
Lane 3 HepG2 cell lysate (20 µg)
Lane 4 Human fetal brain cell lysate (20 µg)
Lanes 1 - 4 Merged signal (red and green). Green - ab171944 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. ab171944 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)](https://www.abcam.com/ps/products/249/ab249663/Images/ab249663-402875-anti-qprt-antibody-epr11941-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)All lanes : Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Jurkat cell lysate
Lane 6 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDaThis data was developed using ab171944, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)](https://www.abcam.com/ps/products/249/ab249663/Images/ab249663-1-anti-qprt-antibody-epr11941-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663) -
![Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)](https://www.abcam.com/ps/products/249/ab249663/Images/ab249663-4-benefits-of-recombinant-antibodies.png)
Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)
