Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11941(B)] to QPRT - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free
    See all QPRT primary antibodies

  • Description

    Rabbit monoclonal [EPR11941(B)] to QPRT - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249663 is the carrier-free version of ab171944. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249663 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)
    Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)

    This data was developed using ab171944, the same antibody clone in a different buffer formulation.

    Lane 1 Wild-type HAP1 cell lysate (20 µg)

    Lane 2 QPRT knockout HAP1 cell lysate (20 µg) 

    Lane 3 HepG2 cell lysate (20 µg)

    Lane 4 Human fetal brain cell lysate (20 µg)

    Lanes 1 - 4 Merged signal (red and green). Green - ab171944 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab171944 was shown to specifically react with QPRT when QPRT knockout samples were used. Wild-type and QPRT knockout samples were subjected to SDS-PAGE. ab171944 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)
    Western blot - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)
    All lanes : Anti-QPRT antibody [EPR11941(B)] (ab171944) at 1/1000 dilution

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human fetal liver tissue lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : Human fetal heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 31 kDa



    This data was developed using ab171944, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)
    Flow Cytometry - Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)
    This data was developed using ab171944, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells labeling QPRT with ab171944 at 1/10 (red) or a rabbit IgG (negative) (green).
  • Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)
    Anti-QPRT antibody [EPR11941(B)] - BSA and Azide free (ab249663)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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