Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HIP2/LIG antibody [EP1145Y] - BSA and Azide free (ab247338)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1145Y] to HIP2/LIG - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HIP2/LIG antibody [EP1145Y] - BSA and Azide free
    See all HIP2/LIG primary antibodies

  • Description

    Rabbit monoclonal [EP1145Y] to HIP2/LIG - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, Jurkat and Daudi cell lysates.

  • General notes

    ab247338 is the carrier-free version of ab52930 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247338 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as HIP2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HIP2/LIG antibody [EP1145Y] - BSA and Azide free (ab247338)
    Western blot - Anti-HIP2/LIG antibody [EP1145Y] - BSA and Azide free (ab247338)
    All lanes : Anti-HIP2/LIG antibody [EP1145Y] (ab52930) at 1/1000 dilution

    Lane 1 : Wild-type HCT116 cell lysate
    Lane 2 : UBEK2 knockout HCT116 cell lysate
    Lane 3 : Daudi cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution

    Predicted band size: 22 kDa



    This data was developed using ab52930, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab52930 observed at 25 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab52930 Anti-HIP2/LIG antibody [EP1145Y] was shown to specifically react with HIP2/LIG in wild-type HCT116 cells. Loss of signal was observed when knockout cell line ab266899 (knockout cell lysate ab257779) was used. Wild-type and HIP2/LIG knockout samples were subjected to SDS-PAGE. ab52930 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-HIP2/LIG antibody [EP1145Y] - BSA and Azide free (ab247338)
    Western blot - Anti-HIP2/LIG antibody [EP1145Y] - BSA and Azide free (ab247338)
    All lanes : Anti-HIP2/LIG antibody [EP1145Y] (ab52930) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : UBE2K knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 22 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab52930).

    Lanes 1-4: Merged signal (red and green). Green - ab52930 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab52930 Anti-HIP2/LIG antibody [EP1145Y] was shown to specifically react with HIP2/LIG in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266031 (knockout cell lysate ab257778) was used.  Wild-type and HIP2/LIG knockout samples were subjected to SDS-PAGE.  ab52930 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-HIP2/LIG antibody [EP1145Y] - BSA and Azide free (ab247338)
    Anti-HIP2/LIG antibody [EP1145Y] - BSA and Azide free (ab247338)

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