Anti-CD90 / Thy1 antibody [EPR3132] - Low endotoxin, Azide free (ab221607)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3132] to CD90 / Thy1 - Low endotoxin, Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-CD90 / Thy1 antibody [EPR3132] - Low endotoxin, Azide free
See all CD90 / Thy1 primary antibodies -
Description
Rabbit monoclonal [EPR3132] to CD90 / Thy1 - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Fetal brain, fetal kidney lysates, Human breast carcinoma, glioma, pancreatic carcinoma and brain tissues.
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General notes
ab221607 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.57 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3132 -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Human pancreatic carcinoma tissue labeling CD90/Thy1 with ab221607 at 1/4000 dilution (0.26 μg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on stromal cells in human pancreatic carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
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ab92574 at 1/100 dilution staining CD90 / Thy1 in (1) Human breast carcinoma, (2) Human glioma & (3) Human brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92574 showing positive staining in Normal human tonsil vessels tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92574 showing positive staining in human Skeletal muscle vessels tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92574 showing positive staining in human Cervical carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab92574 showing positive staining in human Lung adenocarcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92574).
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