Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)
Key features and details
- Rabbit polyclonal to CD45 - Hematopoietic Stem Cell Marker
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD45 antibody - Hematopoietic Stem Cell Marker
See all CD45 primary antibodies -
Description
Rabbit polyclonal to CD45 - Hematopoietic Stem Cell Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig, Monkey
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Immunogen
Synthetic peptide corresponding to Human CD45 aa 1250 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab17550)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab10559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/500. Detects a band of approximately 155 kDa (predicted molecular weight: 147 kDa). IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Target
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Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. -
Involvement in disease
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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Alternative names
- B220 antibody
- CD 45 antibody
- CD45 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)
IHC image of ab10559 staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10559, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)ab10559 rabbit polyclonal to CD45 (1/500) with secondary goat anti-rabbit IgG ab6721 (1/5000).
Expected molecular weight: 147 kDa. WB exposure time: 1min 30sec
Lanes 1 to 10: 20µg of cell lysate per lane
Lanes 6 to 10: ab17550 CD45 blocking peptide used at 1µg/mlLane 1: HeLa Nuclear Extract (ab10559)
Lane 2: HeLa Whole Cell Lysate (ab10559)
Lane 3: A431 Whole Cell Lysate (ab10559)
Lane 4: Jurkat Whole Cell Lysate (ab10559)
Lane 5: HEK293 Whole Cell Lysate (ab10559)
Lane 6: HeLa Nuclear Extract (ab10559+ ab17550)
Lane 7: HeLa Whole Cell Lysate (ab10559+ ab17550)
Lane 8: A431 Whole Cell Lysate (ab10559+ ab17550)
Lane 9: Jurkat Whole Cell Lysate (ab10559+ ab17550)
Lane 10: HEK293 Whole Cell Lysate (ab10559+ ab17550)A strong band, slightly higher was seen in Jurkat cell lysate. The band was blocked using the immunising peptide (ab17550). It is likely that the band is CD45.
Protocols
References (22)
ab10559 has been referenced in 22 publications.
- Coelho S et al. Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells. Sci Adv 6:eaay8271 (2020). PubMed: 32494604
- Kellaway SG et al. Increased proliferation and altered cell cycle regulation in pancreatic stem cells derived from patients with congenital hyperinsulinism. PLoS One 14:e0222350 (2019). PubMed: 31525223
- Li J et al. Co-inhibitory Molecule B7 Superfamily Member 1 Expressed by Tumor-Infiltrating Myeloid Cells Induces Dysfunction of Anti-tumor CD8+ T Cells. Immunity 48:773-786.e5 (2018). PubMed: 29625896
- Wang H et al. The human umbilical cord stem cells improve the viability of OA degenerated chondrocytes. Mol Med Rep 17:4474-4482 (2018). PubMed: 29328479
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)
IHC image of ab10559 staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10559, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)
ab10559 rabbit polyclonal to CD45 (1/500) with secondary goat anti-rabbit IgG ab6721 (1/5000).
Expected molecular weight: 147 kDa. WB exposure time: 1min 30sec
Lanes 1 to 10: 20µg of cell lysate per lane
Lanes 6 to 10: ab17550 CD45 blocking peptide used at 1µg/mlLane 1: HeLa Nuclear Extract (ab10559)
Lane 2: HeLa Whole Cell Lysate (ab10559)
Lane 3: A431 Whole Cell Lysate (ab10559)
Lane 4: Jurkat Whole Cell Lysate (ab10559)
Lane 5: HEK293 Whole Cell Lysate (ab10559)
Lane 6: HeLa Nuclear Extract (ab10559+ ab17550)
Lane 7: HeLa Whole Cell Lysate (ab10559+ ab17550)
Lane 8: A431 Whole Cell Lysate (ab10559+ ab17550)
Lane 9: Jurkat Whole Cell Lysate (ab10559+ ab17550)
Lane 10: HEK293 Whole Cell Lysate (ab10559+ ab17550)A strong band, slightly higher was seen in Jurkat cell lysate. The band was blocked using the immunising peptide (ab17550). It is likely that the band is CD45.
