Anti-CD40 antibody [BLR056F] (ab252557)
![Anti-CD40 antibody [BLR056F] (ab252557)](https://www.abcam.com/ps/products/252/ab252557/Images/ab252557-343248-Rabbit-monoclonal-to-CD40-western-blot-raji-U2OS-daudi-ramos-hdlm2-human.jpg "Anti-CD40 antibody [BLR056F] (ab252557)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR056F] to CD40
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Human
Overview
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Product name
Anti-CD40 antibody [BLR056F]
See all CD40 primary antibodies -
Description
Rabbit monoclonal [BLR056F] to CD40 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide within Human CD40 aa 227-277. The exact sequence is proprietary. NP_001241.1
Database link: P25942-1 -
Positive control
- IHC-P: Human breast carcinoma and tonsil tissue. ICC: Raji cells. WB: Raji, U-2 OS, Daudi, Ramos and HDLM2 whole cell lysate. IP: Raji whole cell lysate.
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General notes
This product is sold under License from Bethyl Laboratories, Inc
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR056F -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD40 antibody (ab252557)All lanes : Anti-CD40 antibody [BLR056F] (ab252557) at 1/1000 dilution
Lane 1 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 4 : GaMG (Human cell line) whole cell lysate
Lane 5 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 6 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 7 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 8 : HDLM2 (Human cell line) whole cell lysate
Lane 9 : RKO (Human cell line) whole cell lysate
Lane 10 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 11 : MJ (Human cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 30 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-CD40 antibody (ab252557)CD40 was immunoprecipitated from Raji (Human Burkitt's lymphoma cell line) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab252557 used for IP at 5 µl per reaction. For WB 1/1000 dilution.Lane 1: ab252557 IP in Raji whole cell lysate.
Lane 2: Control IgG IP in Raji whole cell lysate.Chemiluminescence detection: 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody (ab252557)Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD40 with ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD40 antibody (ab252557)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CD40 with ab252557 at a 1/250 dilution in immunohistochemical analysis. DAB staining. HRP-conjugated goat anti-rabbit IgG secondary.
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![Immunocytochemistry - Anti-CD40 antibody [BLR056F] (ab252557)](https://www.abcam.com/ps/products/252/ab252557/Images/ab252557-343244-Rabbit-monoclonal-to-CD40-immunocytochemistry-raji-human.jpg)
Immunocytochemistry - Anti-CD40 antibody [BLR056F] (ab252557)FFPE section of Raji (Human Burkitt's lymphoma cell line) cells labeling CD40 using ab252557 at a 1/250 dilution in ICC.
Secondary: HRP-conjugated goat anti-rabbit IgG.
Substrate: DAB.
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![Anti-CD40 antibody [BLR056F] (ab252557)](https://www.abcam.com/ps/products/252/ab252557/Images/ab252557-6-benefits-of-recombinant-antibodies.png)
Anti-CD40 antibody [BLR056F] (ab252557)
