Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [1M7.8.1] to CD44 - BSA and Azide free
  • Suitable for: ICC
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD44 antibody [1M7.8.1] - BSA and Azide free
    See all CD44 primary antibodies

  • Description

    Rat monoclonal [1M7.8.1] to CD44 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • ICC: HeLa and NIH/3T3 cells.

  • General notes

    ab272229 is the carrier-free version of ab119348. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab272229 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)
    Immunocytochemistry - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)

    This data was produced using the same clone but in a different formulation, ab119348.

    HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

    Confocal image showing strong membranous staining in HeLa cells.

    Low expression control: MCF7 (PMID: 23039365).

  • Immunocytochemistry - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)
    Immunocytochemistry - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)

    This data was produced using the same clone but in a different formulation, ab119348.

    NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
    Confocal image showing strong membranous staining in NIH/3T3 cells.

  • Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)
    Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)

© 2021 Astana Biomed Group LLP. All rights reserved.