Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)
![Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)](https://www.abcam.com/ps/products/272/ab272229/Images/ab272229-444775-AntiCD44-antibody-1M781-immunocytochemistry-hela-human.jpg "Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1M7.8.1] to CD44 - BSA and Azide free
- Suitable for: ICC
- Reacts with: Mouse, Human
Overview
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Product name
Anti-CD44 antibody [1M7.8.1] - BSA and Azide free
See all CD44 primary antibodies -
Description
Rat monoclonal [1M7.8.1] to CD44 - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species ICC MouseHuman
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Immunogen
Full length protein. This information is considered to be commercially sensitive.
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Positive control
- ICC: HeLa and NIH/3T3 cells.
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General notes
ab272229 is the carrier-free version of ab119348. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab272229 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1M7.8.1 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)
This data was produced using the same clone but in a different formulation, ab119348.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in HeLa cells.
Low expression control: MCF7 (PMID: 23039365).
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![Immunocytochemistry - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)](https://www.abcam.com/ps/products/272/ab272229/Images/ab272229-444774-AntiCD44-antibody-1M781-immunocytochemistry-nih3t3-mouse.jpg)
Immunocytochemistry - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)This data was produced using the same clone but in a different formulation, ab119348.
NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in NIH/3T3 cells. -
![Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)](https://www.abcam.com/ps/products/272/ab272229/Images/ab272229-456243-benefitsofrecombinantantibodies.jpg)
Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)
