Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23102-242] to SAA3 - BSA and Azide free
  • Suitable for: IP, WB, ICC, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free
    See all SAA3 primary antibodies

  • Description

    Rabbit monoclonal [EPR23102-242] to SAA3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-Fr or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse colon, liver, RAW 264.7, J774A.1, NR8383, Rat colon, Rat liver, Rat spleen, His/lif-tagged mouse Saa3 recombinant protein lysates. ICC: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells. IP: Mouse colon tissue lysate.

  • General notes

    ab280893 is the carrier-free version of ab233547. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280893 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23102-242

  • Isotype

    IgG

Images

  • Western blot - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)
    Western blot - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)
    All lanes : Anti-SAA3 antibody [EPR23102-242] (ab233547) at 1/1000 dilution

    Lane 1 : Mouse colon tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse heart tissue lysate
    Lane 5 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 6 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
    Lane 7 : NR8383 (rat norvegicus lung macrophage (alveolar)) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)


    This data was developed using ab233547, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: kidney and heart (PMID:29056547)

    Exposure time: 92 seconds

     

  • Immunocytochemistry - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)
    Immunocytochemistry - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)

    This data was developed using ab233547, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling SAA3 with ab233547 at 1/50 dilution (9.18 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)
    Flow Cytometry - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)

    This data was developed using ab233547, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling SAA3 with ab233547 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)
    Immunoprecipitation - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)

    This data was developed using ab233547, the same antibody clone in a different buffer formulation.

    SAA3 was immunoprecipitated from 0.35 mg of Mouse colon tissue lysate with ab233547 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab233547 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse colon tissue lysate 10 ug

    Lane 2: ab233547 IP in Mouse colon tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab233547 in Mouse colon tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

     

  • Western blot - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)
    Western blot - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)
    All lanes : Anti-SAA3 antibody [EPR23102-242] (ab233547) at 1/1000 dilution

    Lane 1 : Rat colon tissue lysate
    Lane 2 : Rat liver tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : Rat kidney tissue lysate
    Lane 5 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Observed band size: 14 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab233547, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: kidney and heart (PMID:29056547)

    Exposure time: 92 seconds

     

  • Western blot - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)
    Western blot - Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)
    All lanes : Anti-SAA3 antibody [EPR23102-242] (ab233547) at 1/1000 dilution

    Lane 1 : His-tagged mouse Saa1 recombinant protein (aa 20-122) at 0.02 µg
    Lane 2 : E.coli extracts containi his-tagged mouse Saa2 recombinant protein (aa 20-122) at 20 µl
    Lane 3 : His/lif-tagged mouse Saa3 recombinant protein (aa 19-122) at 0.01 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)


    This data was developed using ab233547, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 26 seconds

     

  • Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)
    Anti-SAA3 antibody [EPR23102-242] - BSA and Azide free (ab280893)

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