Anti-CD4 antibody [W3/25] (ab6413)
![Anti-CD4 antibody [W3/25] (ab6413)](https://www.abcam.com/ps/products/6/ab6413/Images/ab6413-1-ab6413antiratCD4spleensplenocytesflowcytometry.jpg "Anti-CD4 antibody [W3/25] (ab6413)")
Key features and details
- Mouse monoclonal [W3/25] to CD4
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Overview
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Product name
Anti-CD4 antibody [W3/25]
See all CD4 primary antibodies -
Description
Mouse monoclonal [W3/25] to CD4 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD4. Rat thymocyte membrane.
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Positive control
- Flow cyt: Lewis rat splenocytes.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
W3/25 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-CD4 antibody [W3/25] (ab6413)
Lewis rat splenocytes stained with ab6413 (right) or mouse IgG1κ (ab170190) isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6413) or mouse IgG1κ (ab170190) (1x106 in 100 µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD3 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.