Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human OX40 ELISA Kit (TNFRSF4) (ab231926)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 23 pg/ml
  • Range: 46.9 pg/ml - 3000 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human OX40 ELISA Kit (TNFRSF4)
    See all CD134 / OX40L receptor kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell medium 5 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Cell medium 3 4.9%

  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    23 pg/ml

  • Range

    46.9 pg/ml - 3000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 120 116% - 122%
    Cell culture extracts 106 102% - 111%
    Cell culture media 113 112% - 114%
    Hep Plasma 120 116% - 121%
    EDTA Plasma 115 112% - 117%
    Cit plasma 116 113% - 117%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human OX40 ELISA Kit (TNFRSF4) (ab231926) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OX40 protein in cit plasma, edta plasma, hep plasma, serum, cell culture supernatant, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human OX40 with 23 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human OX40 ELISA Kit (ab231926)
    Other - Human OX40 ELISA Kit (ab231926)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human OX40 standard curve in Sample Diluent NS
    Example of human OX40 standard curve in Sample Diluent NS

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of human OX40 standard curve in 1X Cell Extraction Buffer PTR
    Example of human OX40 standard curve in 1X Cell Extraction Buffer PTR

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spiked OX40 in human serum and plasma samples
    Interpolated concentrations of spiked OX40 in human serum and plasma samples

    The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 25%, plasma (heparin) 12.5% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    Serum from ten individual healthy human female donors was measured in duplicate. No detectable levels of OX40 were measured.

  • Interpolated concentrations of native OX40 in human cell culture supernatant sample
    Interpolated concentrations of native OX40 in human cell culture supernatant sample

    The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 296 pg/mL in Stimulated PBMC cell culture supernatant, and undetectable in unstimulated and media controls.

  • Interpolated concentrations of native OX40 in human Jurkat Cell Extract, sample based on a 4,000 µg/mL extract load
    Interpolated concentrations of native OX40 in human Jurkat Cell Extract, sample based on a 4,000 µg/mL extract load

    The concentrations of OX40 were measured in duplicate and interpolated from the OX40 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 419 pg/mL in Jurkat Cell Extract.

  • Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926)
    Sandwich ELISA - Human OX40 ELISA Kit (TNFRSF4) (ab231926)
    To learn more about the advantages of recombinant antibodies see here.

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