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Immunology Adaptive Immunity T Cells CD

Anti-CD3 antibody [UCH-T1] - BSA and Azide free (ab215267)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-CD3 antibody [UCH-T1] - BSA and Azide free (ab215267)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [UCH-T1] to CD3 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD3 antibody [UCH-T1] - BSA and Azide free
    See all CD3 primary antibodies
  • Description

    Mouse monoclonal [UCH-T1] to CD3 - BSA and Azide free
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Tissue, cells or virus corresponding to Human CD3. (Human infant thymocytes and Sezary cells).

  • Positive control

    • Molt4 and Jurkat whole cell lysate (ab7899); Human PBMCs.
  • General notes

    Clone UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemias. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin. 

    ab215267 is a recombinant monoclonal antibody produced from the original monoclonal with variable regions (i.e. specificity) from the hybridoma.

Properties

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    UCH-T1
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Images

  • Western blot - Anti-CD3 antibody [UCH-T1] - BSA and Azide free (ab215267)
    Western blot - Anti-CD3 antibody [UCH-T1] - BSA and Azide free (ab215267)
    All lanes : Anti-CD3 antibody [UCH-T1] - BSA and Azide free (ab215267) at 3 µg/ml

    Lane 1 : Jurkat cell lysate (in RIPA buffer)
    Lane 2 : Molt4 cell lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 19 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?



     Primary incubation was 1 hour.

  • Flow Cytometry - Anti-CD3 antibody [UCH-T1] - BSA and Azide free (ab215267)
    Flow Cytometry - Anti-CD3 antibody [UCH-T1] - BSA and Azide free (ab215267)

    Flow cytometric analysis of the dose-dependency (D, F, H) of ab215267 binding to live human PBMCs (B). The gating strategy is shown for control puposes (A, B, C, E, G).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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