Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (ab271997)
![Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (ab271997)](https://www.abcam.com/ps/products/271/ab271997/Images/ab271997-2-ab215527270565215527a.jpg "Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (ab271997)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R1] to Progesterone - BSA and Azide free
- Suitable for: Competitive ELISA
- Reacts with: Species independent
Overview
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Product name
Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free
See all Progesterone primary antibodies -
Description
Rabbit monoclonal [EPPTX-R1] to Progesterone - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Human Progesterone. The immunogen is 11 alpha-Hydroxyprogesterone hemisuccinyl-CH3 BSA.
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Positive control
- Competitive ELISA: Progesterone
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General notes
ab271997 is the carrier-free version of ab215527. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R1 -
Isotype
IgG -
Research areas
Images
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Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (ab271997)
Goat anti-rabbit IgG was coated onto a 96-well plate. Serial dilution of Progesterone (0, 10, 25, 50, 100, 300, 1000 pg/ml) was added (50 μl). This was followed by adding 25 μl HRP-conjugated Progesterone and 3-5 ng/ml of ab215527 (50 μl) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125 μl Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes at room temperature for color development. Then 125 ul of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 10 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215527). -
![Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (ab271997)](https://www.abcam.com/ps/products/271/ab271997/Images/ab271997-1-ab2155272705632155272.jpg)
Competitive ELISA - Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (ab271997)ab215527 is specific to Progesterone and the percent cross reactivity with other compounds is demonstrated in the table.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215527). -
![Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (ab271997)](https://www.abcam.com/ps/products/271/ab271997/Images/ab271997-3-benefits-of-recombinant-antibodies.png)
Anti-Progesterone antibody [EPPTX-R1] - BSA and Azide free (ab271997)
