Anti-CD22 (phospho Y762) antibody [EP727Y] (ab45135)
![Anti-CD22 (phospho Y762) antibody [EP727Y] (ab45135)](https://www.abcam.com/ps/products/45/ab45135/Images/ab45135-415225-anti-cd22-phospho-y762-antibody-ep727y-westernblot-raji-human.jpg "Anti-CD22 (phospho Y762) antibody [EP727Y] (ab45135)")
Key features and details
- Rabbit monoclonal [EP727Y] to CD22 (phospho Y762)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CD22 (phospho Y762) antibody [EP727Y]
See all CD22 primary antibodies -
Description
Rabbit monoclonal [EP727Y] to CD22 (phospho Y762) -
Host species
Rabbit -
Specificity
This antibody will detect CD22 phosphorylation on Tyrosine 762. This antibody is predicted to detect splice isoform 2 based on sequence homology. -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Goat, Chimpanzee, Monkey, Opossum
-
Immunogen
Synthetic peptide within Human CD22. The exact sequence is proprietary.
-
Positive control
- WB: Raji treated with Pervanadate whole cell lysate
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP727Y -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-CD22 (phospho Y762) antibody [EP727Y] (ab45135)All lanes : Anti-CD22 (phospho Y762) antibody [EP727Y] (ab45135) at 1/1000 dilution (Unpurified)
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) treated with Pervanadate whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDa
