Anti-PINCH 1 antibody [EP1944] (ab108609)
![Anti-PINCH 1 antibody [EP1944] (ab108609)](https://www.abcam.com/ps/products/108/ab108609/Images/ab108609-121334-anti-pinch-1-antibody-ep1944-western-blot.jpg "Anti-PINCH 1 antibody [EP1944] (ab108609)")
Key features and details
- Rabbit monoclonal [EP1944] to PINCH 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PINCH 1 antibody [EP1944]
See all PINCH 1 primary antibodies -
Description
Rabbit monoclonal [EP1944] to PINCH 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T, HeLa, Jurkat, Human fetal kidney, and Human spleen cell lysates. IHC-P: Human kidney tissue. ICC: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1944 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PINCH 1 antibody [EP1944] (ab108609)All lanes : Anti-PINCH 1 antibody [EP1944] (ab108609) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH 1 antibody [EP1944] (ab108609)](https://www.abcam.com/ps/products/108/ab108609/Images/ab108609-121333-anti-pinch-1-antibody-ep1944-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH 1 antibody [EP1944] (ab108609)Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab108609 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PINCH 1 antibody [EP1944] (ab108609)](https://www.abcam.com/ps/products/108/ab108609/Images/ab108609-121332-anti-pinch-1-antibody-ep1944-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PINCH 1 antibody [EP1944] (ab108609)Immunofluorescent staining of HeLa cells using ab108609 at 1/100 dilution.
