Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22412-229] to CD200 / OX2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD200 / OX2 antibody [EPR22412-229]
    See all CD200 / OX2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22412-229] to CD200 / OX2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain, lung, placenta and tonsil tissue lysate. IHC-P: Human tonsil and placenta tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)
    Western blot - Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)
    All lanes : Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human lung tissue lysate
    Lane 3 : Human placenta tissue lysate
    Lane 4 : Human tonsil tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

    Predicted band size: 31 kDa
    Observed band size: 40-50 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure Time: Lane 1: 7 secs; Lane 2: 3 mins; Lane 3: 3 secs; Lane 4: 3 mins.

    The molecular weight observed is consistent with literatures (PMID: PMID:20070425, PMID:18256920, PMID:18938162).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling CD200 / OX2 with ab254193 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 11260322) is observed. The section was incubated with ab233345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD200 / OX2 with ab254193 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 11260322) is observed. The section was incubated with ab233345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)
    Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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