Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Wednesday March 03, 2021

Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22412-229] to CD200 / OX2
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-CD200 / OX2 antibody [EPR22412-229]
See all CD200 / OX2 primary antibodies
Description

Rabbit monoclonal [EPR22412-229] to CD200 / OX2
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human brain, lung, placenta and tonsil tissue lysate. IHC-P: Human tonsil and placenta tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22412-229
Isotype

IgG
Research areas

Western blot - Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)

All lanes : Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Human tonsil tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

Predicted band size: 31 kDa
Observed band size: 40-50 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure Time: Lane 1: 7 secs; Lane 2: 3 mins; Lane 3: 3 secs; Lane 4: 3 mins.

The molecular weight observed is consistent with literatures (PMID: PMID:20070425, PMID:18256920, PMID:18938162).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling CD200 / OX2 with ab254193 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 11260322) is observed. The section was incubated with ab233345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD200 / OX2 with ab254193 at 1/1000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 11260322) is observed. The section was incubated with ab233345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-CD200 / OX2 antibody [EPR22412-229] (ab254193)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com