This data was developed using the same antibody clone in a different buffer formulation (ab33734)

C57BL/6 mouse splenocytes stained with ab33734 (right) or Rat IgG2aκ (ab18450) isotype (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in 1x PBS / 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab33734) or Rat IgG2aκ (ab18450) (1x¹⁰⁶ in 100 µl at 0.2 µg/ml) for 30 min on ice.

The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ^® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD19 antibody.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.