Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Mouse monoclonal [4B4F12] to CD14
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Human
Isotype: IgG1

Product name

Anti-CD14 antibody [4B4F12]
See all CD14 primary antibodies
Description

Mouse monoclonal [4B4F12] to CD14
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CD14 aa 1-250. Expressed in E. coli.
Database link: P08571

Run BLAST with Run BLAST with
Positive control
- Human CD14 recombinant protein (amino acids 20-214), lysate from HEK293 cells transfected with CD14 (amino acids 20-214)-hIgGFc, HepG2, A549, HL60, mouse RAW264.7, Hela, HEK293 and mouse NIH 3T3 cell lysates. Jurkat cells. Human stomach cancer tissue.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

4B4F12
Isotype

IgG1
Research areas

Flow Cytometry - Anti-CD14 antibody [4B4F12] (ab182032)

Flow cytometric analysis of Jurkat cells labeling CD14 using ab182032 at 1/200 (green) and a negative control (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [4B4F12] (ab182032)

Immunohistochemical analysis of paraffin-embedded, DAB-stained Human stomach cancer tissues labeling CD14 using ab182032 at 1/200.
Western blot - Anti-CD14 antibody [4B4F12] (ab182032)

Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution + Human CD14 recombinant protein (amino acids 20-214)

Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [4B4F12] (ab182032) This image is courtesy of an anonymous abreview.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue section labeling CD14 with ab182032. The tissue was fixed with formaldehyde, heat mediated antigen retrieval was performed followed by blocking with 5% serum for 12 hours at 4°C. The tissue was incubated with undiluted Anti-CD14 antibody [4B4F12] (ab182032) for 12 hours at 4°C.
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Western blot - Anti-CD14 antibody [4B4F12] (ab182032)

All lanes : Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution

Lane 1 : Lysate from HEK293 cells
Lane 2 : Lysate from HEK293 cells transfected with CD14 (amino acids 20-214)-hIgGFc

Predicted band size: 40 kDa
Western blot - Anti-CD14 antibody [4B4F12] (ab182032)

All lanes : Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : mouse RAW264.7 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : HEK293 cell lysate
Lane 7 : mouse NIH 3T3 cell lysate

Predicted band size: 40 kDa

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