Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse/Rat CNTF ELISA Kit (ab270889)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 10.75 pg/ml
  • Range: 140.63 pg/ml - 9000 pg/ml
  • Sample type: Cell culture media, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Mouse/Rat CNTF ELISA Kit
    See all CNTF kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 1.1%

  • Sample type

    Serum, Cell culture media

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    10.75 pg/ml

  • Range

    140.63 pg/ml - 9000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 95% - 99%
    Cell culture media 96 92% - 101%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse, Rat

  • Product overview

    Mouse/Rat CNTF ELISA kit (ab270889) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CNTF protein in mouse/rat serum and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate mouse/rat CNTF with 10.75 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Mouse and Rat Ciliary neurotrophic factor (CNTF) is a single chain polypeptide of 22.7 kDa. CNTF is a member of a limited family of neuropoietic cytokines that also includes Leukemia Inhibitory Factor and Oncostatin M. This family of cytokines are characterized by four distinctive α-helical folds commonly associated with growth hormone. CNTF has been hypothesized to be a target-derived differentiation factor for developing neurons and glial cells. CNTF that is retained intracellularly has been suggested to be a lesion-associated molecule, providing damaged neurons with trophic or survival support following injury. Mouse and rat CNTF have 95% sequence identity and the standard protein in this product is the mouse sequence.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of mouse/rat CNTF standard curve in Sample Diluent NS.
    Example of mouse/rat CNTF standard curve in Sample Diluent NS.

    The CNTF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spiked CNTF in mouse/rat serum and cell culture supernatant samples.
    Interpolated concentrations of spiked CNTF in mouse/rat serum and cell culture supernatant samples.

    The concentrations of CNTF were measured in duplicates, interpolated from the CNTF standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse serum 12.5%, rat plasma 6.25%, and 10F RPMI cell culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Mouse/Rat CNTF ELISA Kit (ab270889)
    Sandwich ELISA - Mouse/Rat CNTF ELISA Kit (ab270889)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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