Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD133 antibody [CMab-43] - BSA and Azide free (ab264545)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [CMab-43] to CD133 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD133 antibody [CMab-43] - BSA and Azide free
    See all CD133 primary antibodies

  • Description

    Mouse monoclonal [CMab-43] to CD133 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human CD133. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: O43490

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Caco-2, HT-29 and NCCIT whole cell lysate. IHC-P: Human pancreas tissue. Human endometrium carcinoma tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab264545 is the carrier-free version of ab264538. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-CD133 antibody [CMab-43] - BSA and Azide free (ab264545)
    Western blot - Anti-CD133 antibody [CMab-43] - BSA and Azide free (ab264545)
    All lanes : Anti-CD133 antibody [CMab-43] (ab264538) at 1/1000 dilution

    Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : NCCIT (human pluripotent embryonic carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 97 kDa
    Observed band size: 128 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The 128KDa band is glycosylated form which is  consistent with what has been described in the literature (PMID: 28910211, 20199106, 30873590).

    Exposure time: 7 seconds.

    This image was produced using the same antibody clone but in a different formulation ab264538, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [CMab-43] - BSA and Azide free (ab264545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [CMab-43] - BSA and Azide free (ab264545)

    Immunohistochemical analysis of paraffin-embedded human endometrium carcinoma tissue labeling CD133 with ab264538 at 1/2000 dilution followed by a ready to use secondary. Apical staining on ducts of human endometrium carcinoma. The section was incubated with ab264538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264538).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [CMab-43] - BSA and Azide free (ab264545)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [CMab-43] - BSA and Azide free (ab264545)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CD133 with ab264538 at 1/2000 dilution followed by a ready to use secondary. Apical staining on ducts of human pancreas. The section was incubated with ab264538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264538).

  • Anti-CD133 antibody [CMab-43] - BSA and Azide free (ab264545)
    Anti-CD133 antibody [CMab-43] - BSA and Azide free (ab264545)

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