Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free (ab238658)
![Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free (ab238658)](https://www.abcam.com/ps/products/238/ab238658/Images/ab238658-354092-Mouse-monoclonal-OX42-to-CD11b-c--BSA-and-Azide-free-immunocytochemistry-nr8383-rat-macrophages.jpg "Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free (ab238658)")
Key features and details
- Mouse monoclonal [OX42] to CD11b + CD11c - BSA and Azide free
- Suitable for: ELISA, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free -
Description
Mouse monoclonal [OX42] to CD11b + CD11c - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Rat
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Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: NR8383 cells, rat macrophages.
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General notes
ab238658 is a PBS-only buffer format of ab1211.
IHC protocol advice:
This antibody is not suitable for IHC on paraffin-embedded samples.
For best results in IHC on frozen tissue, the following may help detection:
1) Paraformaldehyde perfusion fixed samples have worked well for many customers.
2) For non-perfused tissue, either snap freeze or emerse in periodate-lysine-paraformaldehyde (PLP) fixative for 24 hours at 4°C. Reduce the concentration of paraformaldehyde to 0.25-0.5% since this increases the staining intensity for immune cell surface markers (PMID: 7868861).
3) PFA-fixed samples will require cryoprotection by sucrose infiltration.
4) Use OCT (TissueTek) compound for embedding.
5) For snap frozen tissue, fix sections in cold acetone for 10 min. Allow to dry for 10 min at room temperature. Wash with water for 10 min.
6) Do not heat the samples or sections.
7) During the staining procedure, do not allow the sections to dry out.Our Technical team (technical@abcam.com) will be happy to provide further information and advice.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX42 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free (ab238658)
NR8383 cells (rat macrophage cell line) stained for CD11b + CD11c (shown in green) using ab1211 in ICC/IF. The cells were fixed with 100% methanol for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3 M glycine in 0.1% Tween-PBS for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1211 at 5µg/ml) overnight at +4°C. The secondary antibody was ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (shown in red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (shown in blue) at a concentration of 1.43 µM for 1 hour at room temperature.
This image was produced using the same antibody clone but in a different formulation; PBS, arginine and sodium azide (ab1211).
