Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20681] to CYR61/CCN1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free
    See all CYR61/CCN1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20681] to CYR61/CCN1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: MDA-MB-231 cells. WB: MDA-MB-231, MCF7 and HeLa cell lysates. Flow Cyt: MDA-MB-231 and MCF7 cells. IP: Saos-2 cell lysate.

  • General notes

    Ab233097 is the carrier-free version of ab230947. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab233097 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)
    Western blot - Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)
    All lanes : Anti-CYR61/CCN1 antibody [EPR20681] (ab230947) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CYR61 knockout HeLa cell lysate
    Lane 3 : MDA-MB-231 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab230947).

    Lanes 1-4: Merged signal (red and green). Green - ab230947 observed at 47 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab230947 Anti-CYR61/CCN1 antibody [EPR20681] was shown to specifically react with CYR61/CCN1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265288 (knockout cell lysate ab257406) was used.  Wild-type and CYR61/CCN1 knockout samples were subjected to SDS-PAGE.  ab230947 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)
    Immunoprecipitation - Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)

    CYR61/CCN1 was immunoprecipitated from 0.35 mg Saos-2 (human osteosarcoma epithelial) whole cell lysate with ab230947 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230947 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
    Lane 1: Saos-2 (human osteosarcoma epithelial) whole cell lysate 10 µg (Input).
    Lane 2: ab230947 IP in Saos-2 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230947 in Saos-2 whole cell lysate (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 8 seconds.

    The molecular mass observed is consistent with that of the full-length protein (42 kDa) (PMID: 23798676).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230947).

  • Flow Cytometry - Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)
    Flow Cytometry - Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell, Left) / MDA-MB-231 (human breast adenocarcinoma epithelial cell, Right) cell line labeling CYR61/CCN1 with ab230947 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative control: MCF7 (PMID: 11059746).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230947).

  • Immunocytochemistry/ Immunofluorescence - Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)
    Immunocytochemistry/ Immunofluorescence - Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) cells labeling CYR61/CCN1 with ab230947 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the MDA-MB-231 cell line. Negative control: MCF7 (PMID: 11059746). The nuclear counter stain is DAPI (blue).
    Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230947).

  • Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)
    Anti-CYR61/CCN1 antibody [EPR20681] - BSA and Azide free (ab233097)

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