Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19911-220] to CD105 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-CD105 antibody [EPR19911-220] - BSA and Azide free
    See all CD105 primary antibodies

  • Description

    Rabbit monoclonal [EPR19911-220] to CD105 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLA whole cell lysate. IHC-P: Human liver and kidney tissue; rat kidney tissue.

  • General notes

    Ab252548 is the carrier-free version of ab252345. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab252548 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CD105 with ab252345 at 1/2000 dilution, followed by the Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on peritubular microvasculature of rat kidney (PMID: 25381426) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab252345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252345).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD105 with ab252345 at 1/2000 dilution, followed by the Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on sinusoidal endothelial cells of human liver (PMID: 30563158) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab252345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252345).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD105 with ab252345 at 1/2000 dilution, followed by the Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on glomerular and peritubular microvasculature of human kidney (PMID: 25381426) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes.

    The section was incubated with ab252345 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252345).

  • Immunoprecipitation - Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)
    Immunoprecipitation - Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)

    CD105 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab252345 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab252345 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input). 
    Lane 2: ab252345 IP in HeLa whole cell lysate. 
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252345 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252345).

  • Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)
    Anti-CD105 antibody [EPR19911-220] - BSA and Azide free (ab252548)

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