Anti-CD105 antibody [105C02] (ab44967)
![Anti-CD105 antibody [105C02] (ab44967)](https://www.abcam.com/ps/products/44/ab44967/Images/ab44967-341545-ab44967KOWBGDB.jpg "Anti-CD105 antibody [105C02] (ab44967)")
Key features and details
- Mouse monoclonal [105C02] to CD105
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD105 antibody [105C02]
See all CD105 primary antibodies -
Description
Mouse monoclonal [105C02] to CD105 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Purified GP160 from cell membrane glycoproteins of fresh non-T/non-B acute lymphoblastic leukemia cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
105C02 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-CD105 antibody [105C02] (ab44967)All lanes : Anti-CD105 antibody [105C02] (ab44967) at 1/1000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : CD105 knockout HeLa whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Observed band size: 95 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab44967 observed at 95 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab44967 was shown to specifically react with CD105 in wild-type HeLa cells as signal was lost in CD105 knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab44967 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
