Human ENG (CD105) knockout HeLa cell line (ab265178)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Cervix -
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10 -
Antibiotic resistance
Puromycin 1.00µg/ml -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human ENG knockout HeLa cell line (ab265178)All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution
Lane 1 : HeLa WT
Lane 2 : ENG HeLa KO
Lane 3 : HUVEC
Lane 4 : MCF7
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 70-120 kDa why is the actual band size different from the predicted?ab170943 Anti-CD105 antibody [EPR10145-10] was shown to specifically react with CD105 in HeLa wild type cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild type and CD105 knockout samples were subjected to SDS-PAGE. ab170943 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human ENG knockout HeLa cell line (ab265178)All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution
Lane 1 : HeLa WT
Lane 2 : ENG HeLa KO
Lane 3 : HUVEC
Lane 4 : MCF7
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 70-120 kDa why is the actual band size different from the predicted?ab169545 Anti-CD105 antibody [EPR10145-12] was shown to specifically react with CD105 in HeLa wild type cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild type and CD105 knockout samples were subjected to SDS-PAGE. ab169545 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human ENG knockout HeLa cell line (ab265178)Allele-1: 19 bp deletion in exon 2.
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Sanger Sequencing - Human ENG knockout HeLa cell line (ab265178)Allele-2: 11 bp deletion in exon 2.
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Sanger Sequencing - Human ENG knockout HeLa cell line (ab265178)Allele-3: 1 bp insertion in exon 2.