Anti-CCR5 antibody [EPR23449-327] - BSA and Azide free (ab280204)
![Anti-CCR5 antibody [EPR23449-327] - BSA and Azide free (ab280204)](https://www.abcam.com/ps/products/280/ab280204/Images/ab280204-2-anti-ccr5-antibody-epr23449327-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg "Anti-CCR5 antibody [EPR23449-327] - BSA and Azide free (ab280204)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23449-327] to CCR5 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CCR5 antibody [EPR23449-327] - BSA and Azide free
See all CCR5 primary antibodies -
Description
Rabbit monoclonal [EPR23449-327] to CCR5 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: Human PBMC cells; Human PBMC cells treated with 50 ng/ml PMA and 500 ng/ml lonomycin for 24 hours.
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General notes
ab280204 is the carrier-free version of ab254377. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280204 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23449-327 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CCR5 antibody [EPR23449-327] - BSA and Azide free (ab280204)
This data was developed using ab254377, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) labelling CCR5 with ab254377 at 1/50 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab254377. Then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells.
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![Flow Cytometry - Anti-CCR5 antibody [EPR23449-327] - BSA and Azide free (ab280204)](https://www.abcam.com/ps/products/280/ab280204/Images/ab280204-3-anti-ccr5-antibody-epr23449327-flow-cytometry-human-peripheral-blood-mononuclear-cell1.jpg)
Flow Cytometry - Anti-CCR5 antibody [EPR23449-327] - BSA and Azide free (ab280204)This data was developed using ab254377, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) (treated with 50 ng/ml PMA and 500 ng/ml lonomycin for 24 hours) labelling CCR5 with ab254377 at 1/50 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab254377. Then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells.
